1. Classify media, detail common ingredients of culture media.
2. Define Sterilisation?Discuss principle, classify methods, working and applications of autoclave.
3. Construction and design of an aseptic area.
4. Microbiology? It's scope and application, history
5. Ultrastructure of bacteria? Note on flagella? Cell wall?
6. Define stain? Classification, type.
7. Assessment of microbial contamination and spoilage.
8. Microbial assay, assay of Streptomycin by cup plate method.
9. Cultivation of virus.
10. Aseptic techniques, laminar air flow system.
11. Evaluation of bactericidal, static, disinfectants.
12. Phenol as disinfectant
13. Explain gram staining, difference.
14. Techniques of preservation of pure culture.
15. Scanning electron microscopy.
16. Difference between flagella and pilli.
17. Contribution of Louise pasteurs.
18. Lytic growth cycle of bacteriophage.
19. Microbial assay of antibiotics by cup plate method.
20. Explain replication in virus.
21. Factors affecting microbial spoilage.
22. Growth curve in detail of bacteria.
23. Source of contamination and preservation in aseptic area.
24. Classify fungi? And its reproduction.
25. Define microbiology, give its history, scope and importance.
26. Explain Sterility testing of pharmaceutical product as per IP.
27. Write a note on laminar air flow.
28. Explain sources of contamination in aseptic area.
29. Write note on physical method of sterilisation.
30. Write short note on hot air oven.
